Asian Journal of Bio Science

P. D. Jogi ¹, R. D. Dharmale ², M. S. Dudhare ¹, A. A. Aware ²

Affiliations
1 Department of Agricultural Biotechnology, Vasantrao Naik College of Agriculture Biotechnology, Yavatmal(M.S.), IN
2 Department of Zoology, Brijlala Biyani Science College, Amravati (M.S.), IN

Abstract

A water treated with magnet is called as magnetic water. Magnetic water when applied to normal water, restructure the water molecules into very small water molecules clusters, each made up of six symmetrically organized molecules. This miniscale cluster is recognized by cell as bio-friendly due to its hexagonal structure, because toxins can't travels within the cluster and easily enter the passageways in plant and animal cell membrane. The result provides maximum healthy hydration with less water for the preparation of magnetic water (South pole energy increased tubes with north pole energy produced larger above ground plant). Magnetic water is useful to promote plant growth; in which 30 per cent growth occurs. It facilated the plant growth as magnetic field neutralize water element. Treated Seedlings markly improve imbibitions and germination of seed. Seedling treatment promotes NPK absorption and increases the stem thickness, increases leaf lamina as well as increases tiller numbers, pod size. Irrigated plant with magnetic water increased chlorophyll content, nitrate reductase activity and reduced permeability of cell membrane at low temperature.

Keywords

Magnet, Agriculture, Growth, Mustard Seeds (Brassica nigra L.), Magnetic Water.

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S. P. Hadke ¹, A. G. Deshmukh ², M. S. Dudhare ², E. R. Vaidya ²

Affiliations
1 Department of Botany, Agnihotri College of Science, Wardha (M.S.), IN
2 Biotechnology Centre, Department of Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN

Abstract

Simarouba glauca is an oil yielding tree with a very high productivity and its rapid multiplication is essential for mitigating the edible/industrial oil demand. For developing in vitro regeneration protocol, optimization of surface sterilization of explant and study of effect of auxins and cytokinins is essential. The explant was exposed to mercuric chloride (0.1 %) and Sodium hypochloride (1 %) for a period of 2-12 minutes. Both the surface sterilizing agents gave near about same results with no contamination even at lowest contact time, however, as the contact time was increased intense browning was observed. The callus induction was observed at all the concentration of 2,4-D and BAP. More callusing was observed with higher concentration of both 2,4 D and BAP.

Keywords

Simarouba glauca, Callus, Auxins, Cytokinins.

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M. S. Dudhare ¹, S. K. Katiyar ²

Affiliations
1 Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), IN
2 Department of Biotechnology, Indira Gandhi Agricultural University, Raipur (C.G.), IN

Abstract

A double haploid mapping population consisting of 75 lines of a cross between the irrigated indica variety IR64 and the upland japonica varietyAzucena (Guiderdoni et al., 1992) was used in the present experiment. The complete set of double haploid lines along with parents was evaluated for molecular mapping. A set of 264 microsatellite markers was used for parental polymorphism screening of which 60 markers were found polymorphic. Thirty-one of these markers were associated with QTLs of different traits and are distributed on eight different rice chromosomes. These markers can be successfully used to transfer some of the QTLs into different genetic backgrounds.

Keywords

QTLs, Rice, Drought.

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M. S. Dudhare ¹, A. G. Deshmukh ²

Affiliations
1 Department of Biotechnology, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), IN
2 Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), IN

Abstract

Food crises in the sixties led to the development of high yielding varieties of cereals particularly, rice and wheat which resulted into green revolution and food production was increased significantly. A rapidly expanding population is a serious concern for food security especially for developing countries including that of India. To keep pace with the present population growth and consumption pattern, India’s food requirement has been estimated to 225 million tons by the year 2005. Green revolution technologies, though contributing to food production, they are inadequate to meet the challenges lie ahead. Recently developed biotechnological techniques undoubtedly have major role to play in solving the food security problem, just as the green revolution had its origins in science and technology, and particularly in science of genetics. So the application of novel biotechnological tools could lead to gene revolution. With the wide spread of advanced cellular and gene technology, the new technologies will certainly help in enhancing the crop productivity.

Keywords

Somaclones, Transgenic, Gene Technology, Food Crises, Productivity.

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M. S. Dudhare ¹, A. G. Deshmukh ¹

Affiliations
1 Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), IN

Abstract

In Lactic streptococci several metabolic properties which are vital for successful dairy fermentation are unstable. With the advent of techniques for studying genetic composition of dairy streptococci, it has become possible to provide explanation for this unstable phenomenon. These organisms characteristically harbor many plasmid species (AkCelik, 1999). The number observed ranges from two to eleven, but most strains appear to contain four to seven distinct plasmid species.

Keywords

Subculturing, Metabolic, Activities, Srains Lactococcus Lactic.

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